Spatiotemporal properties of intracellular calcium signaling in osteocytic and osteoblastic cell networks under fluid flow

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Spatiotemporal properties of intracellular calcium signaling in osteocytic and osteoblastic cell networks under fluid flow.

Mechanical stimuli can trigger intracellular calcium (Ca(2+)) responses in osteocytes and osteoblasts. Successful construction of bone cell networks necessitates more elaborate and systematic analysis for the spatiotemporal properties of Ca(2+) signaling in the networks. In the present study, an unsupervised algorithm based on independent component analysis (ICA) was employed to extract the Ca(...

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Osteocytes compose 90-95% of all bone cells and are the mechanosensors of bone. In this study, the strain experienced by individual osteocytes resulting from an applied fluid flow shear stress was quantified and correlated to two biological responses measured in real-time within the same individual osteocytes: (1) the upregulation of intracellular calcium and (2) changes in intracellular nitric...

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Intracellular calcium signaling.

In excitable cells, the major pathway for Ca2+ influx is via highly Ca2+-selective voltage-gated Ca2+ channels (VGCC). The original alphabetical nomenclature was replaced recently with standard numerical nomenclature: Cav1.1-1.4, Cav2.1-2.3, Cav3.1-3.3. In skeletal muscle cells, an additional voltagesensitive pathway is provided by dihydropyridine receptors (DHPR, Cav1.1) that do not pass signi...

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ژورنال

عنوان ژورنال: Bone

سال: 2013

ISSN: 8756-3282

DOI: 10.1016/j.bone.2013.01.008